Figure 5. qRT-PCR validation of RNA-seq targets. HRMECs were pre-treated with the vehicle or GSK0660 for 24 h, followed by stimulation with 1 ng/ml TNFα for 4 h. The mRNA expression was evaluated by RNA-seq and qRT-PCR. The fold change for RNA-seq was determined by the edgeR algorithm, while the fold change for qRT-PCR was determined by the comparative Ct method and it is relative to the β-actin expression levels. All fold changes are relative to HRMECs treated with the vehicle alone. Error bars indicate standard deviation for three samples in each group. *p = 0.0003, **p<0.0001.