Figure 6. The inhibitory effect of PD98059 and LY294002 on TCS-401-induced proliferation and migration in RPE cells. A: Inhibition of TCS-401-induced proliferation by pretreatment with 10 μM LY294002 and 20 μM PD98059 for 30 min, as measured
with MTT after 24 h incubation. B: Inhibition of TCS-401-induced migration by pretreatment with 10 μM LY294002 and 20 μM PD98059 for 30 min, as measured with
a transwell assay after 12 h incubation. Original magnification=100X. C: The number of migrated cells per HPF was shown. The data represent the mean±standard deviation of three independent experiments.
*p<0.05, compared to the control group. #p<0.05, compared to the treatment with only TCS-401. HPF, high-power field; RPE, retinal pigment epithelium.