Figure 3. Effect of TSA on keratocyte phenotypes in compressed collagen matrices. Maximum intensity projection images of f-actin organization in corneal keratocytes following 4 days of culture within compressed collagen matrices. A: Keratocytes in compressed collagen matrices cultured in basal serum-free media (S-) with vehicle developed a dendritic morphology with membrane-associated f-actin labeling, and this morphology was maintained in the presence of TSA (B). C: PDGF BB induced keratocyte spreading in compressed collagen matrices without inducing stress fiber formation, and this spreading response was maintained in the presence of TSA (D). E: TGFβ1 induced the loss of dendritic processes and the formation of stress fibers, and this transformation was blocked by TSA (F). G: Left side of graph shows cell length following 4 days of culture inside compressed ECM. *PDGF induced a significant increase in cell length as compared to basal media and TGFβ, both with and without TSA (p<0.05, two way ANOVA). #In both basal media and TGF1β, cell length was greater in the presence of TSA (p<0.05, two way ANOVA). Right side of graph shows breadth/length following 4 days of culture. #Cells cultured in TGFβ1 were less elongated than cells cultured in TGFβ1 + TSA (p<0.05, two way ANOVA). Measurements were performed on a minimum of 16 cells for each condition, taken from three separate experiments.