Figure 5. H&E stained lens bow regions. Examples of each fixation routine are presented, labeled as described in Materials and Methods.
Acetone fixation (C) has a clearly negative effect on the lens, with capsular separation and severe shrinkage. As seen in D, inconsistent separation between capsule and lens mass can occur, though this was not common. Formalin induces some lens
shrinkage and the emergence of perinuclear shrinkage (black arrow, G).