Figure 10. Counting of cell sides. CellProfiler output images for counting the number of neighboring cells. A: Cells that are not touching the border of the image and are within a specified size range. All cell measurements reflect cells within a specific size range that do not touch the border of an image. This image excludes these border elements. B: A second identification of cells was performed to obtain accurate counts for cells that neighbored cells touching the border. This identification included all cells (and all partial cells), independent of their location within the image. The number of neighboring cells for the cells next to border cells was the only statistic that was altered through this second round of identification.