Figure 7. MMP-2, MMP-9, VEGFA, CTGF, VEGFR-1, cathepsin D, TIMP-1, TIMP-2, and α-SMA gene expression levels in RPE cell cultures treated with anti-CTGF antibody. RPE cell cultures treated for 48 h with anticonnective tissue growth factor (anti-CTGF) 10 µg/ml and without anti-CTGF as control, and then RNA extraction and real-time RT–PCR were performed as described in the Methods section. mRNA levels were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and presented as folds of the control values. Anti-CTGF decreased gene expression of matrix metalloproteinase-2 (MMP-2) compared to the control. *p<0.05.