Figure 7. Dose-responsive effect of ATP on [Ca²⁺]_i in fhRPE cells. A: Represented by normalized change in percent fluorescence, the responses in [Ca²⁺] to 5 µM ATP are shown for control fhRPE and fhRPE overexpressing hBest1 or hBest1^W93CB: Dose response curves for the initial rise in [Ca²⁺]_i as a function of ATP concentration are shown for control monolayers and for monolayers overexpressing wild-type or mutant hBest1. ATP doses were between 1 and 100 µM. Differences in responses between each group were significant by ANOVA (p<0.003, n≥5). Data are mean ± SEM.