Figure 2. Localization of endogenous hBest1, overexpressed hBest1, and overexpressed hBest1^W93C in fhRPE cells. Monolayers of fhRPE were stained for hBest1 expression (green) and examined by confocal microscopy in the X-Y (A, C, E) and X-Z (B, D, F) planes. Endogenous hBest1 (A, B) was observed to be localized to the basolateral plasma membrane, as was hBest1 in monolayers overexpressing hBest1 (C, D) or hBest1^W93C (E, F). Nuclei (red) were stained for positional referencing. Scale bars: 20 µM.