Figure 1. Whole-cell chloride currents mediated by Best1-CFP, Best1-YFP, and Best1^W93C-YFP. A: Whole-cell patch-clamp recordings were performed on HEK293 cells transfected with Best1-CFP, Best1-YFP, or Best1-CFP and Best1-YFP. Cells expressing fluorescently tagged Best1 exhibited robust chloride currents compared to untransfected cells recorded from the same plate. B: In contrast, Best1^W93C exhibited a significant loss of anion channel activity compared to Best1-YFP (B), and dominantly inhibited the anion channel activity of wild-type Best1-YFP when co-expressed (C).