Figure 4. Effect of miR-21 on mRNA and protein expression of α-SMA in orbital fibroblasts from TAO donors (n=3). A: Real-time PCR analysis of the mRNA level of α-smooth muscle actin (α-SMA). B: Western blot analysis of the protein level of α-SMA. C: Quantification of the protein band density in panel B. Results are expressed as mean ± standard deviation (SD; *p<0.05 versus control). Data are representative of three independent experiments.