Table 1 of
Sahu, Mol Vis 2015; 21:273-284. Table 1 of
Sahu, Mol Vis 2015; 21:273-284.
Table 1. PCR primer for genotyping Wt wt/wt,Wtrd8/rd8, Ctrp5+/−,rd8/rd8 mice and for amplification of rd8 locus for sequencing.
| Gene | Allele | Primer name | Sequence (5′-3′) |
|---|---|---|---|
| Crb1 | Wild-type | 3663-mCrb1 mF1 | GTGAAGACAGCTACAGTTCTGATC |
| 3665-mCrb1 mR | GCCCCATTTGCACACTGATGAC | ||
| Crb1 | Mutant | 3664-mCrb1 mF2 | GCCCCTGTTTGCATGGAGGAAACT TGGAAGACAGCTACAGTTCTTCTG |
| 3665-mCrb1 mR | GCCCCATTTGCACACTGATGAC | ||
| Crb1 | rd8 | 3666-Crb-F | GGTGACCAATCTGTTGACAATCC |
| 3667-Crb-R | GCCCCATTTGCACACTGATGAC | ||
| Ctrp5 | Knock-in | 2855-Ctrp5-F | CCCCTACCTTTCGACCGT |
| 2856-Ctrp5-R | GAAGAAAGAGGCGATGGACTG |