Figure 2. Autofluorescence SLO fundus images of L-ORD mice lacking the rd8 mutation. A: Representative autofluorescent-scanning laser ophthalmoscopy (AF-SLO) images of C57BL/6 wild-type control mice free of the
rd8 mutation (Wtwt/wt) and late-onset retinal degeneration (L-ORD) mice free of the rd8 mutation (Ctrp5+/−;wt/wt). The Wtwt/wt mice showed few AF spots by 21 months. However, the AF spots were significantly increased in the Ctrp5+/−;wt/wt mice by 21 months of age. Magnification bar=200 μm. B: Quantification of the AF spots in Wtwt/wt (black triangle) and Ctrp5+/−;wt/wt (black diamond) per the AF-SLO images. The number of AF spots is shown as the mean number of spots ± SD. There was an age-related
increase in AF spots (linear regression plot) in both genotypes. However, the AF spots accumulated to a high number in L-ORD
mice by 21 months when compared to the age-matched control mice (p<0.001).