Table 1 of
Kumari, Mol Vis 2015; 21:26-39. Table 1 of
Kumari, Mol Vis 2015; 21:26-39.
Table 1. Details of primers used for expression analysis.
| S.No | Name of the gene | NCBI Id | Sequence | Annealing temperature/cycles |
|---|---|---|---|---|
| 1. | αA- cry | NM_012534.2 | Sense: TGGCAAACACAACGAGAGGCA Antisense: GCCAGCATCCAAGCCAGACT | 58 °C/35 |
| 2. | βB1 -cry | NM_012936.2 | Sense: CACCAACACAGCATCAGAAACCAT Antisense: GTTTTCCTGCTCAAAGACGACCA | 60 °C/35 |
| 3. | γD- cry | NM_033095.1 | Sense primer: CGACTCTGTCCGCTCCTGCC Antisense: TACTCCCCGGGCCTCAGCAG | 59 °C/35 |
| 4. | Cat | NM_012520.1 | Sense: ACTGGGACCTCGTGGGAAAC Antisense: TCTGGAATCCCTCGGTCGCT | 57 °C/40 |
| 5. | SOD-1 | NM_017050.1 | Sense: CGGTGCAGGGCGTCATTCACTT Antisense: CTCTTCATCCGCTGGACCGCC | 58 °C/35 |
| 6. | Gpx | NM_030826.3 | Sense: CACCACGACCCGGGACTACA Antisense: AGGTAAAGAGCGGGTGAGCC | 58 °C/35 |
| 7. | Fas | NM_139194.2 | Sense: TTCGAATGCAAGGGACTGAT Antisense: TGGGCCCACTTGATATAACC | 56 °C/35 |
| 8. | TNF-α | NM_012675.2 | Sense: TAGCAAACCACCAAGCAGAG Antisense: CCTTGAAGAGAACCTGGGAG | 60 °C/40 |
| 9. | Caspase-8 | NM_022277.1 | Sense: TGGGAAGGATCGACGATTAC Antisense: CCACATGTCCTGCATTTTGA | 58 °C/35 |
| 10. | Caspase-3 | NM_012922.2 | Sense: GGTTCATCCAGTCACTTTGC Antisense: CCAGGGAGAAGGACTCAAAT | 60 °C/40 |
| 11. | Bid | NM_022684.1 | Sense: CTAGGGACATGGAGAACGAC Antisense: CCCTCACGTAGGAGAATAGG | 60 °C/40 |
| 12. | Bad | NM_022698.1 | Sense: TCCGAAGAATGAGCGATGAA Antisense: TTCCCCAAATTTCGATCCCA | 59 °C/40 |
| 13. | Bax | NM_017059.2 | Sense: CGATGAACTGGACAACAACA Antisense: AGCAAAGTAGAAAAGGGCAA | 60 °C/40 |
| 14. | Bcl-2 | NM_016993.1 | Sense: GGGATGCCTTTGTGGAACTA Antisense: CTCACTTGTGGCCCAGGTAT | 60 °C/35 |
| 15. | Caspase-9 | NM_031632.1 | Sense: GAACGACCTGACTGCTAAGA Antisense: GAACGACCTGACTGCTAAGA | 57 °C/35 |
| 16. | Cytochrome- C | JF919282.1 | Sense: TTGTTCAAAAGTGTGCCCAG Antisense: GTAAGAGAATCCAGCAGCCT | 61 °C/40 |
| 17. | NFkB | NM_001276711.1 | Sense: ACACCTCTACACATAGCAGC Antisense: GGCACTACTCCCTCATCTTC | 60 °C /40 |
| 18. | β-actin | NM_001109553.1 | Forward primer: AGTCCCTCACCCTCCCAAAA Reverse primer: AGCAATGCTGTCACCTTCCC | 60 °C /40 |