Figure 4. Repression of human PRR 3′UTR by miR-152. hRECs were co-transfected with WT (A) or mutant (B) PRR 3′-UTRs and 20 nM of scrambled miRNA (NC), miR-152 mimics, or 50 nM miR-152 antagomirs using the Lipofectamine™ 2000 transfection reagent (Invitrogen, Carlsbad, CA). Luciferase activity was determined 24 h after transfection. Values are the means ± SEM of relative luciferase activity over NC after normalization to the Renilla luciferase activity from two independent experiments in triplicate.