Figure 7. Calcein AM assay of TM cells following treatment with β-amyloid, naloxone, and CD44 Ab. A: Trabecular meshwork (TM) cells were treated with β-amyloid 1–42 (200 ng) and naloxone alone or in combination B: TM cells were treated with 0.1, 1, and 10 µg CD44 neutralizing antibody (CD44 Ab). Error bars are presented as ± standard error of the mean (SEM), ***p<0.01 with respect to PBS (control), † p<0.05 comparing β-amyloid versus β-amyloid plus naloxone, ‡ p<0.05 comparing 0.1 µg CD44Ab to 1 µg CD44Ab, §§§p<0.0001 comparing 0.1 µg CD44Ab to 10 µg CD44Ab, #p<0.05 comparing 1 µg CD44Ab to 10 µg CD44Ab.