Figure 6. Wound healing rate is decreased in diabetic limbal epithelial cells compared to healthy ones as revealed by scratch wound assay in the presence of 1 and 4 ng/ml epidermal growth factor. A: Wound healing in the presence of 1 and 4 ng/ml EGF was evaluated by measuring wound areas for healthy (NL) and diabetic (DM) LEC using ImageJ software and presented as percentage of wound area for healthy (NL) and diabetic (DM) LEC using ImageJ software and presented as percentage of wound area coverage ± standard error of mean. * p<0.05; *** p<0.0001 versus healthy controls. B: Representative images of healthy and diabetic limbal epithelial cells (LECs) after 20 h of wound healing in the medium with 1 ng/ml epidermal growth factor (EGF). Vertical lines demarcate the borders of the original wound. Bar=500 µm.