Figure 5. Both keratins 15 and K17 are expressed at substantially lower levels in limbal epithelial cells isolated from diabetic corneas than from healthy corneas. Immunostaining for K15 (A, B) and K17 (E, F) is decreased in diabetic (DM) LEC as compared to the healthy (NL) ones. Images in A and B, or in E and F were obtained using the same exposure time. C, D, same pictures as in A and B, and G, H are the same as E and F, respectively, but with 4',6-diamidino-2-phenylindole (DAPI) nuclear counterstain. Cells were cultured on fibronectin, collagen type IV, and laminin (FCL). Bars=20 μm.