Figure 4. Stem cell marker ABCG2 expression is decreased in the cytoplasm of diabetic limbal epithelial cells as compared to their healthy counterparts. Images in A and B represent healthy (NL) and diabetic (DM) LEC and were obtained using the same exposure time. C, D, same pictures as in A and B, respectively, but with 4',6-diamidino-2-phenylindole (DAPI) nuclear counterstain. Cells were cultured on fibronectin, collagen type IV, and laminin (FCL). Bar=20 μm.