Table 2 of Forest, Mol Vis 2015; 21:1345-1356.

Table 2. Evaluation of immunostaining at different temperatures with methanol and paraformaldehyde fixation.

Target proteins Methanol 0.5% paraformaldehyde
−20 °C RT 37 °C 50 °C 4 °C RT 37 °C 50 °C
ZO-1 ++++ +++ ++ + ++++ +++ ++ +
hnRNP L ++ +++ ++ + ++ ++++ ++++ +
actin + +++ ++++ ++ - - - -
histone H3 - ++ +++ ++++ D ++++ ++++ -

-: null; +: weak, ++: medium; +++: good; ++++: excellent; D: detachment of endothelial cells by sodium dodecyl sulfate; RT: room temperature.

Forest, Mol Vis 2015; 21:1345-1356. http://www.molvis.org/molvis/v21/1345

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