Figure 1 of Forest, Mol Vis 2015; 21:1345-1356.

Figure 1. Schematics of the experiments designed for optimizing immunostaining on flatmounted whole corneas. Four fixation temperatures were assessed for each of the two fixatives. Only the main steps are presented. For details, refer to the Methods section. *Antigen retrieval using sodium dodecyl sulfate (SDS) was used only for revealing histone H3. room temperature=RT.