Figure 3. Nidogen-2 immunocytochemistry of corneal stromal cells under different culture conditions. The expression of nidogen-2 protein (green) is seen to be highest in the perinuclear organelle, whereas in myofibroblasts nidogen-2 protein expression appears as a meshwork-like pattern throughout the cell body (white arrowheads). Fibroblasts have little, if any, nidogen-2 protein expression, except the perinuclear organelle staining, as was observed in keratocytes. Isotypic control immunoglobulin (IgG) staining was negative under all cell conditions, as shown for the myofibroblasts. Magnification=400X.