Figure 8. Collagen IV protein levels are reduced in KC corneas. The representative photomicrographs show staining for COL IV (Brown color) with counterstain hematoxylin (blue color) for nuclei. The staining is observed in the basement membrane and immediate sub-epithelium, as indicated by the arrows. Three independent representative samples for KC and control corneas are presented at 40X magnification. Scale bar: 10 µm.