Figure 4. TGF-β1 and TGF-β2 mRNA investigated with RT–PCR (continuous 48 h) in serum-free conditions. Ranibizumab regulated transforming growth factor-β1 (TGF-β1) mRNA expression in vitro. Human Tenon’s fibroblasts (HTFs) treated with ranibizumab concentration 0.5 mg/ml demonstrated significant decrease in the mRNA level of TGF-β1 (p<0.05; n=3). However no significant changes distinguished the mRNA level of TGF-β2 (p<0.05; n=3).