Figure 2. Effects of anti-miR-155 on VEGF-induced HRMEC proliferation, migration, and tube formation. VEGF-induced cell proliferation was inhibited in the anti-miR-155 treatment group at both 24 h and 48 h compared with the control groups (VEGF-treated control and anti-ctrl group; A). B shows representative images (10⁻⁶ mg/ml agents) of various treatment groups and the results of the statistical analysis. The cell nuclei were stained with DAPI, which is indicated in blue. Compared to the control group, the VEGF-induced migratory activities of the anti-miR-155 groups were inhibited (B). A Matrigel assay was used to evaluate tube formation. The total length of each tube in all treatment groups is presented. C shows the results of the statistical analysis and representative images of the various treatment groups. The tube formation study demonstrated that anti-miR-155 inhibited the effects of VEGF-induced angiogenesis. Each experiment was repeated at least three times. Data are presented as mean ± SD. *p<0.05, **p<0.01; ***p<0.0001. Scale bar: 20 μm.