Table 4 of Le Guezennec, Mol Vis 2015; 21:1151-1161.

Table 4. Level of four cytokines in 1000 patients pooled tear sample at 5 or 10 µl volume.

Analytes Experiment 1 (5 µl) # Experiment 2 (5 µl) #   Experiment 3 (10 µl) ##
Mean±SD
(pg/ml) Intra-assay %CV N Mean±SD
(pg/ml) Intra-assay %CV N Inter-assay %CV Mean±SD (pg/ml) Intra-assay %CV N
IL-6 12.93±-1.42 19.58 3 9.19±0.86 9.41 3 20.84 15.81±0.45 2.86 2
TNF-α 1.03±-0.13 12.73 3 1.47±0.27 18.14 3 10.47 2.47±0.29 12 2
IL-1β NA+ NA+ 0+ 1.49±0.45 30.37 22+ NA 2.4±0.08 3.23 2
IFN-γ 5.53±-0.98 17.62 3 5.87±0.39 6.78 3 12.04 9.23±0.7 7.58 2

#5 µl tear sample/5 µl beads reaction condition ## 10 µl tear sample/5 µl beads reaction condition. +IL-1β below detectable level. . 2+IL-1β with one well out of three wells below detectable level. Linear Correlation between results of 5 µl (Experiment 2) and 10 µl tears (Experiment 3) indicate R2=0.98

Le Guezennec, Mol Vis 2015; 21:1151-1161. http://www.molvis.org/molvis/v21/1151

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