Table 1 of Le Guezennec, Mol Vis 2015; 21:1151-1161.

Table 1. Summary of precision and accuracy determination with four cytokine standards.

Analytes Experiment 1 Experiment 2 Inter-assay %CV LOD
pg/ml
Detection range(LLOQ-ULOQ) pg/ml Intra- Assay
%CV R2 Detection range(LLOQ-ULOQ) pg/ml Intra- Assay
%CV R2
IL-6 2.41–2501.65 5.04 1.00 2.40–9820–03 1.18 1.00 9.71 1.5
TNF-α 2.47–2496.87 4.58 1.00 2.43–2505.28 3.17 1.00 6.00 0.78
IL-1β 4.98–16024.06 0.7 0.99 4.93–17882.88 5.15 1.00 8.41 1.19
IFN-γ 10.06–37464.21 4.38 1.00 9.87–38786.96 3.82 1.00 8.44 2.66

Detection range was determined with Bio-Plex manager software. LOD was calculated as the mean +2 SD of the blank in both experiments. Intra-assay %CV: Intra-assay precision for each individual experiment. Inter-assay %CV: interassay precision of both experiments. R2 was calculated by correlating measured concentration to expected concentration of standards.

Le Guezennec, Mol Vis 2015; 21:1151-1161. http://www.molvis.org/molvis/v21/1151

©2015 Molecular Vision http://www.molvis.org/molvis/

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