Figure 2. Five-parameter logistic curve of DA-Bead. Curves from experiment 2 are presented in this figure. The blue dotted line represents the lower limit of quantitation (LLOQ) and the upper limit of quantitation (ULOQ) calculated with Bio-Plex Manager software. FitProb and ResVar are the fitting probability and the residual variance, respectively, calculated by Bio-Plex Manager software for the indicated curve. The concentration and the median fluorescence intensity (MFI) scale are in the log range.