Figure 3. Detection of KCC2b, STEP, and GABAAβ3 receptor subunit proteins in the young adult rabbit lens. A–C: Striatal enriched tyrosine phosphatase (STEP) mAb (green) and anti-KCC2b (red) immunofluorescence signals, with the merged images shown in panel C (200X). D–F: STEP (green) and KCC2b are present at the fiber cell borders in the fiber cells indicated by the asterisk in the panels above (600X, rabbit fiber cells are about 10 μm across in the longest dimension shown). Arrows identify corresponding site in panels D–F. Panels G–I demonstrate KCC2b expression and GABAAβ3 receptor subunit (green) in mature fiber cells corresponding to the site indicated by the asterisk in panels above.