Figure 1. Morphology and characterization of rCjECs. Light microscopy showed the morphology of the rabbit conjunctival epithelial cells (rCjECs). A: Cells appear to be compact, uniform, and the typical cobblestone shape. Phalloidin staining for visualization of rCjEC morphology. Goblet cells contain secretory vesicles in the cytoplasm (the arrowhead indicates goblet cells). B: Mucins were detected with periodic acid–Schiff (PAS) staining (goblet cells are indicated by the arrowhead). C: Immunocytochemistry was performed to observe conjunctival epithelial-specific markers. D: CK4 for non-goblet cells. E: CK19 for non-goblet cells. F: MUC5AC for goblet cells. Nuclei were counterstained with 4',6-diamidino-2-phenylindole dihydrochloride (DAPI). Scale bars: 100 μm.