Table 1 of
Smit-McBride, Mol Vis 2015; 21:110-123. Table 1 of
Smit-McBride, Mol Vis 2015; 21:110-123.
Table 1. Antibodies, suppliers, and concentrations used for immunohistochemistry and western blots.
| Primary Antibody and Concentration Used (Manufacturer & catalog #) | Host Species | Immunogen | Secondary Antibody (Manufacturer & catalog #) |
|---|---|---|---|
| anti-mouse Factor H, affinity purified polyclonal, 2 ug/ml (IHC-P), 1 ug/ml (WB); (Santa Cruz Biotechnology, catalog # 17951) | goat | Peptide mapping within an internal region of Factor H of mouse origin (aa 250–300) | Biotinylated bovine anti-goat IgG (Jackson ImmunoResearch, catalog # 805–065–180) Cy3 donkey anti-goat IgG (Jackson ImmunoResearch, catalog # 705–165–147) Alkaline Phosphatase conjugated bovine anti-goat IgG (Jackson ImmunoResearch, catalog #805055180) |
| anti-mouse Factor H, recombinant polyclonal, 6 ug/ml (IHC-P), 1 ug/ml (WB), (Santa Cruz Biotechnology, catalog # 33157) | rabbit | Amino acids 61–360 mapping within an internal region of Factor H of mouse origin | Biotinylated donkey anti-rabbit IgG (Jackson ImmunoResearch, catalog # 711–065–152) Alkaline Phosphatase goat anti-rabbit IgG (Jackson ImmunoResearch, catalog #111055003) |
| anti-Rhodopsin, clone 4D2, monoclonal antibody, 1:500, (Millipore, catalog # MABN15) | mouse | Rat rod outer segments corresponding to rat Rhodopsin at the N-terminus | Cy2 conjugated donkey anti-mouse IgG, 1:100 (Jackson ImmunoResearch, catalog #715–225–150) |
| anti-Opsin, Blue, polyclonal, 1:500, (Millipore catalog # AB5407) | rabbit | Recombinant human blue opsin | Cy5 conjugated donkey anti-rabbit IgG, 1:100 (Jackson ImmunoResearch, catalog #711–175–152) |
| anti-Opsin, Red/Green, 1:500, polyclonal, (Millipore catalog # AB5405) | rabbit | Recombinant human red/green opsin | Cy5 conjugated donkey anti-rabbit IgG, 1:100 (Jackson ImmunoResearch, catalog #711–175–152) |