Table 1 of
Itakura, Mol Vis 2015; 21:1071-1084. Table 1 of
Itakura, Mol Vis 2015; 21:1071-1084.
Table 1. Oligonucleotides used in this study
| Site-Specific Mutagenesis | ||
|---|---|---|
| MYOC mutation | Direction | Oligonucleotide sequence (3′) |
| Y437H | Sense | ATCATCTGTGGCACCTTGCACACCGTCAGCAGCTACACCT |
| Antisense | AGGTGTAGCTGCTGACGGTGTGCAAGGTGCCACAGATGAT | |
| Q368X | Sense | CCTGGAGCTGGCTACCACGGATAGTTCCCGTATTCTTGGG |
| Antisense | CCCAAGAATACGGGAACTATCCGTGGTAGCCAGCTCCAGG | |
| A427T | Sense | ATCCGTAAGCAGTCAGTCACCAATGCCTTCATCATCTGTG |
| Antisense | CACAGATGATGAAGGCATTGGTGACTGACTGCTTACGGAT | |
| RT–PCR | ||
| Gene | Direction | Oligonucleotide sequence (5’-3′) |
| IL1A | Sense | ATCAGTACCTCACGGCTGCT |
| Antisense | TGGGTATCCCAGGCATCTCC | |
| IL1B | Sense | CTGAAAGCTCTCCACCTCCA |
| Antisense | TCATCTTTCAACACGCAGGA | |
| IL6 | Sense | CACACAGACAGCCACTCACC |
| Antisense | TTTCACCAGGCAAGTCTCCT | |
| TGFB2 | Sense | CGCCAAGGAGGTTTACAAAA |
| Antisense | TGCTGAGACGTCAAATCGAA | |
| FOXO1 | Sense | AAGAGCGTGCCCTACTTCAA |
| Antisense | CACCCTCTGGATTGAGCATC | |
| ACTA2 | Sense | ATGCCTCTGGACGTACAACTG |
| Antisense | CGGCAGTAGTCACGAAGGAAT | |