Figure 2. Expression of Wild Type and Mutant MYOCs by Stably Transduced Cell Lines MYOC expression was induced in TM-1 cell lines stably transduced with wild-type or mutant MYOC lentivirus expression constructs across a range of doxycycline concentrations (0, 100, and 1000 ng/ml). After 4 days time, conditioned media and cell lysates were collected for assay. Lanes of an SDS-polyacrylamide gel were equally loaded with aliquots of each sample (20 ul of cell culture media or 20 ug cell lysates) and proteins were separated by electrophoresis. Western blotting was performed with MYOC antibody to visualize MYOC proteins. Blots were re-probed with antibodies against ACTB as a control for equal loading of gel lanes. The electrophoretic mobility of molecular weight size standards is indicated.