Figure 5. VNUT expression in the glaucomatous and control retinas at different ages (3, 9, 15, and 22 months). A: Detection of vesicular nucleotide transporter (VNUT) (68 kDa) levels in the C57BL/6J mice at 3, 9, and 15 months and in the DBA/2J mice at 3, 9, 15, and 22 months by western blotting. The glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 37 kDa) levels are shown as a loading control. B: Western blot analysis of the VNUT at 3, 9, 15, and 22 months of age. Values are the mean ± standard deviation (SD) of six independent animal experiments (***p<0.001).