Figure 3. rGal-1 modulates L-selectin and β2-integrin. Peripheral leukocytes were labeled with antibodies specific for the surface adhesion
molecules CD62L (conjugated with PE) and CD11b (conjugated with fluorescein isothiocyanate [FITC]). A-C: Representative dot plots of cells (each point represents a cell) immunostained for adhesion molecules. A: Control, B: LPS, C: LPS + recombinant galectin-1 (rGal-1), D: percentages of CD62L-positive cells, E: CD11b-positive cells, and F: CD62L/CD11b-positive cells. Data represent the median ± SEM of percentage of cells. *p<0.05, versus control; ##p<0.01 versus
LPS. G: Representative immunoblot demonstrates the levels of CD11b and CD62L in the pooled extracts of rat eyes (n = 5 animals/group)
from the control, LPS, and LPS + rGal-1 groups at 24 h (data illustrate one representative of two independent experiments).
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as loading control.