Detection of VEGF levels by ELISA in HLE-B3 cells treated with SB216763 +/− hypoxia inducible factor translation inhibitors.
HLE-B3 cells were cultured in 25 cm2
flasks with 20% fetal bovine serum (FBS) and switched to serum-free media for 24 h before the experiment. We used the cell
culture medium from the cell populations described in Figure 3
. At the end of 3 h of drug exposure, the cell culture medium was analyzed for vascular endothelial growth factor (VEGF).
The experiment was repeated twice using independent cell populations stemming from a single cell passage. The cell culture
medium was evaluated for vascular endothelial growth factor (VEGF) levels using six samples from two independent cell populations.
The asterisk (*) signifies there was a statistically significant increase in the VEGF levels. Resulting data indicated that
the level of VEGF in the SB216763-treated samples was substantially higher than that of the non-treated control samples (p<0.05).
In both cases, SB216763-treated samples with supplemented hypoxia-inducible factor-1α (HIF-1α) translation inhibitor or HIF-2α
translation inhibitor showed the same propensity to be elevated above the control levels. SB=SB216763. HIF-1α=hypoxia inducible
factor-1 translation inhibitor. HIF-2α=hypoxia inducible factor-2 translation inhibitor.