Figure 5. Effects of atropine treatment on collagen type I and Regulator of G-protein signaling 2 (RGS2) expression in the sclera of form deprivation myopia (FDM) eyes. Real-time-PCR analysis of collagen type I (A) and RGS2 (C) mRNA expression levels in the sclera of guinea pigs. Data were analyzed by the 2^–ΔΔC_T method and normalized to internal control β-actin as described in the Methods (n = 10 samples in each group). Western blots of collagen type I (B) and RGS2 (D) protein expression levels in the sclera of guinea pigs (top panels), analyzed by measuring band density and normalized by the internal control β-tubulin (n = 10 samples in each group). mRNA and protein expression levels were normalized and expressed as fold change (ratio of atropine group to saline group). Data shown are mean±SEM. Statistical analysis was performed on the mean data using a Student t test, *p<0.05 compared to the saline control.