**Figure 4. **Effects of atropine treatment on scleral collagen production. **A**: Representative photomicrographs of scleral sections of H&E staining from the contralateral control and form deprivation
myopia (FDM) eyes with or without atropine treatment. **B**: Quantitative analysis of the thickness of the sclera by Soft Cell^{A} Imaging Software (n = 10 eyes in each group, six sections from each eye). Each point represents mean±SEM. Statistical analysis
was performed on the mean data from each guinea pig using a Student *t* test, *p<0.05 compared to the saline control. Arrows indicate the sclera. Scale bar is 50 µm.