Figure 3. Regulator of G-protein signaling 2 (RGS2) upregulation in the sclera induced by form deprivation (FD). A: Real-time PCR analysis of RGS2 mRNA expression level in guinea pig sclera. Data were analyzed by the 2^–ΔΔC_T method and normalized to β-actin as described in the Methods (n = 10 samples in each group). B: Western blot analysis of RGS2 protein expression level in guinea pig sclera (inset), analyzed by measuring band density and normalized by β-tubulin (n = 10 samples in each group). mRNA and protein expression levels were normalized and expressed as fold change (ratio of treated group to normal eye group). Data shown are mean±SEM. Statistical analysis was performed on the mean data using a Student t test, *p<0.05 compared to the control.