Figure 5. Dilution of MAGEA3-producing bacteria in nonrelevant bacteria. A: After induction of protein expression by Isopropyl β-D-thiogalactoside (IPTG), MAGEA3-producing bacteria were mixed with
Melan-A-producing bacteria, opsonized with complement, and incubated overnight with human leukocyte antigen (HLA)-DP4 Epstein-Barr
virus-transformed B cells (EBV-B cells), which were generated from blood cells of the Vogt-Konayagi-Harada (VKH) patient.
The anti-MAGE-3 T cell clone (5,000 cells/well) was added to the antigen-presenting cells (30,000 cells/well). The amount
of interferon (IFN)-γ secreted in the supernatant of overnight co-cultures was measured by enzyme-linked immunosorbent assay
(ELISA). The results shown represent an average of triplicate co-cultures. B: EBV-B.retro-Ii.MAGEA3 cells and HLA-DP4 EBV-B cells loaded with Melan-A-expressing bacteria were used as positive and negative
controls, respectively. Error bars represent SD.