Figure 3. Identity of the recombinant glutathione-S-transferase (GST)-fusion proteins produced in bacteria. Each protein of interest produced in fusion with GST was detected with a relevant specific antibody. The western blot profiles show a variety of recombinant protein fragments as previously detected by the monoclonal anti-GST antibody, as shown in Figure 2. (-): bacterial extracts from induced culture of bacteria containing the empty vector pKE-1. (+): bacterial extracts from induced cultures of recombinant bacteria containing the sequence encoding GST-fusion proteins.