Figure 3. Ionized calcium-binding adaptor molecule 1 immunostaining on flatmounted retinas. A–B: Example of flatmounted retinas from rat eyes with endotoxin-induced uveitis (EIU) injected with vehicle (A) or with antivascular endothelial growth factor (VEGF) antibodies (B); Bar = 50 µm. Arrowhead show round reactive ionized calcium-binding adaptor molecule 1 (IBA1)-positive cells. C–D: Extraction of cell contours used for automatic labeling of IBA1 staining. E-F: Quantification of round and total IBA-1 positive cells on flat-mounted retina from EIU control and anti-VEGF treated eyes. Bar = 50 µm.