Figure 6. Diabetes does not change the interaction of GluA2 subunit with GRIP1 or NSF in the retina. Diabetes (2, 4, or 8 weeks’ duration) was induced by a single intraperitoneal injection of streptozotocin in Wistar rats. The protein interaction between GluA2 subunit and its interacting proteins was analyzed with coimmunoprecipitation followed by western blotting. A: Immunoreactivity of GRIP1 and NSF in the immunoprecipitated samples of GluA2. B: Immunoreactivity of GluA2 in the immunoprecipitated samples of GRIP1. C: Immunoreactivity of GluA2 in the immunoprecipitated samples of NSF. The results are presented as the mean ± standard error of the mean (SEM) of at least three independent experiments (at least six retinas from three animals) and are expressed as a percentage of the control. Representative immunoblots are presented at the right of the respective graphs.