Figure 4. Effect of diabetes on the composition of AMPA receptors in the retina. Diabetes (2, 4, or 8 weeks’ duration) was induced by a single intraperitoneal injection of streptozotocin in Wistar rats. Th interaction between AMPA receptor subunits was analyzed with coimmunoprecipitation (IP) followed by western blotting (WB). A: Immunoreactivity of GluA2 and GluA4 in the immunoprecipitated samples of GluA1. B: Immunoreactivity of GluA1 and GluA4 in the immunoprecipitated samples of GluA2. C: Immunoreactivity of GluA1 and GluA2 in the immunoprecipitated samples of GluA4. The results are presented as the mean ± standard error of the mean (SEM) of at least three independent experiments (at least six retinas from three animals) and are expressed as a percentage of the control. Representative immunoblots are presented at the right of the respective graphs. *p<0.05, ***p<0.001, significantly different from the control as determined with the Student t test.