Figure 3. Nitrite production in cultured macrophages incubated with subretinal fluid from eyes with retinopathy of prematurity and retinal detachment. A: Subretinal fluid (SRF) from eyes with retinopathy of prematurity (ROP) induced robust production of nitrite at levels similar to lipopolysaccharide (LPS; positive control; Wilcoxon signed-rank test, - p ≥ 0.05, *p < 0.05, **p < 0.001). SRF from eyes with retinal detachment (RD) did not have any measurable effects. B: Detailed comparison of nitrite production between every two different conditions, for instance, p > 0.05 between macrophage and medium, p < macrophage + LPS 1 μg/ml versus medium.