Figure 1. Effects of subretinal fluid from eyes with retinopathy of prematurity and retinal detachment on the proliferation of human retinal microvascular endothelial cells; evaluation of toxic effect of subretinal fluid from patients with retinal detachment on human retinal microvascular endothelial cells. A: Data were normalized to the mean values of the human retinal microvascular endothelial cells (HRMECs) grown in medium alone. Subconfluent cultures were incubated with 10% concentrations of subretinal fluid (SRF) from eyes with retinopathy of prematurity (ROP) and retinal detachment (RD). SRF from ROP eyes induced an increase in the proliferation effect while SRF from RD eyes showed a modest inhibitory effect. Vascular endothelial growth factor A (VEGF-A; 10–100 ng/ml) was used as the positive control. B: The dead cell rates (the dead cell rate=the number of dead cells/the number of (dead cells + live cells) x 100%) of HRMECs cultured in different conditions.