Figure 2. Fluorescence microscopy with red fluorescent antibody for integrin α5 in normal and laser-treated rat RPE-choroid. The red arrows show integrin α5 expression near normal blood vessels. The yellow arrows show positive red antibody binding for integrin α5 between the subretinal and suprachoroidal spaces with ruptured Bruch's membranes after laser treatment. The white arrow indicates isolectin binding in green macrophages and microglial and endothelial cells and near new tubular structures (green arrows). The blue arrows show colocalization of red integrin α5 with green macrophages and microglial and endothelial cells. Blue 4',6-diamidino-2-phenylindole (DAPI) nuclear stain highlights the disruption and wound healing in the inner and outer nuclear layers. Scale bar = 100 μm.