Table 1 of
Reis, Mol Vis 2014; 20:836-842. Table 1 of
Reis, Mol Vis 2014; 20:836-842.
Table 1. Primers and conditions for EPHA2, CRYBB3 and CRYBA2 PCR amplification.
| Gene | Primers (5′-3′) | PCR size (bp) | Special conditions |
|---|---|---|---|
| EPHA2 | F: CAGCGACATCAAGAGGATTG | 285 | Betaine solution; annealing at 60 °C |
| R: TGGTCATCTCCTCAGTTCAG | |||
| CRYBA2 | F: AGTGAGGCAGAGGATGTATC | 367 | FailSafe Mix D; annealing at 60 °C |
| R: TCCAGTCTGACTCCATCATC | |||
| CRYBB3 | F: TGTAGGCAGGCAGAGTGCAT | 501 | Betaine solution; annealing at 58 °C |
| R: AGTCTCAGAACACTCAAGC |