Figure 3. VEGF-A₁₆₅b inhibited VEGF-A₁₆₅a-ICAM-1 expression. A: Immunoblot for ICAM 1 expression in primary RPE cells. B: Relative expression of the intercellular adhesion molecule-1 (ICAM-1) protein. ICAM-1 was normalized with α-tubulin (as the internal control) and expressed as a percentage of the control group. Data are expressed as the mean ± standard error of the mean (SEM) of three independent experiments; ***p<0.001 compared with the control, ###p<0.001 compared with VEGF-A₁₆₅a treated alone.