Figure 2. VEGF-A₁₆₅a-induced ICAM in primary RPE cells. A: Immunoblot for ICAM1 expression. The effect was inhibited by anti- vascular endothelial growth factor (VEGF)-A₁₆₅a and VEGFR-2 inhibitor, ZM323881. B: Relative expression of the intercellular adhesion molecule (ICAM-1) protein. ICAM-1 was normalized to α-tubulin and expressed as a percentage of the control group. Data are expressed as the mean ± standard error of the mean (SEM) of three independent experiments; ***p<0.001 compared with the control, ###p<0.001 compared with 100 ng/ml VEGF-A₁₆₅a-treated group.