Figure 7. ARPE-19 cells were transfected with p62 and Nrf2 siRNA for 24 h followed by 24 h pretreatment of cells with 5 µM pinosylvin
and finally 24 h oxidative stress evoked with 400 µM hydroquinone (HQ). Cell viability was measured in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT) test. Data are expressed as the mean value±standard deviation (SD), n = 5. *p<0.05, significantly different
from cells treated with TR and HQ, ANOVA, and the t test. TR=transfection agent; PS=pinosylvin; HQ=hydroquinone; Neg. siRNA=negative siRNA.